期刊
EMBO JOURNAL
卷 29, 期 10, 页码 1659-1673出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2010.54
关键词
heterochromatin; nuclear compartmentalization; nuclear pore complex; Tpr
资金
- Swedish Research Council
Amassments of heterochromatin in somatic cells occur in close contact with the nuclear envelope (NE) but are gapped by channel- and cone-like zones that appear largely free of heterochromatin and associated with the nuclear pore complexes (NPCs). To identify proteins involved in forming such heterochromatin exclusion zones (HEZs), we used a cell culture model in which chromatin condensation induced by poliovirus (PV) infection revealed HEZs resembling those in normal tissue cells. HEZ occurrence depended on the NPC-associated protein Tpr and its large coiled coil-forming domain. RNAi-mediated loss of Tpr allowed condensing chromatin to occur all along the NE's nuclear surface, resulting in HEZs no longer being established and NPCs covered by heterochromatin. These results assign a central function to Tpr as a determinant of perinuclear organization, with a direct role in forming a morphologically distinct nuclear sub-compartment and delimiting heterochromatin distribution. The EMBO Journal (2010) 29, 1659-1673. doi: 10.1038/emboj.2010.54; Published online 20 April 2010
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