期刊
EMBO JOURNAL
卷 29, 期 19, 页码 3344-3357出版社
WILEY
DOI: 10.1038/emboj.2010.210
关键词
cell cycle; centrosome; Drosophila; mitosis; proteomics
资金
- BL laboratory: Berliner Senat fur Kultur
- MB laboratory: DFG
- BL laboratory: EFRE
- BL laboratory: NGFN2 SMP Protein
- BL laboratory: NGFN Plus
- BL laboratory: EU
- BL laboratory: Wissenschaft und Forschung
- MB laboratory: European Commission
- MB laboratory: HFSP
- BL laboratory: IG Mutanom
- JG laboratory: Technologiestifiung Berlin (TSB)
- JG laboratory: European Union
- JG laboratory: NGFN2 SMP Protein
- AP laboratory: Leibniz Association
- AP laboratory: Joint Initiative for Research and Innovation
Regulation of centrosome structure, duplication and segregation is integrated into cellular pathways that control cell cycle progression and growth. As part of these pathways, numerous proteins with well-established non-centrosomal localization and function associate with the centrosome to fulfill regulatory functions. In turn, classical centrosomal components take up functional and structural roles as part of other cellular organelles and compartments. Thus, although a comprehensive inventory of centrosome components is missing, emerging evidence indicates that its molecular composition reflects the complexity of its functions. We analysed the Drosophila embryonic centrosomal proteome using immunoisolation in combination with mass spectrometry. The 251 identified components were functionally characterized by RNA interference. Among those, a core group of 11 proteins was critical for centrosome structure maintenance. Depletion of any of these proteins in Drosophila SL2 cells resulted in centrosome disintegration, revealing a molecular dependency of centrosome structure on components of the protein translation machinery, actin- and RNA-binding proteins. In total, we assigned novel centrosome-related functions to 24 proteins and confirmed 13 of these in human cells. The EMBO Journal (2010) 29, 3344-3357. doi:10.1038/emboj.2010.210; Published online 3 September 2010
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