期刊
EMBO JOURNAL
卷 29, 期 17, 页码 2930-2942出版社
WILEY
DOI: 10.1038/emboj.2010.158
关键词
cyclin-dependent kinase; polarized exocytosis; polarized growth; Spitzenkorper
资金
- BBSRC [BB/H003126/1]
- Yorkshire Cancer Research [S304]
- Wellcome Trust [GR077544AIA]
- Biotechnology and Biological Sciences Research Council [BB/E003273/1] Funding Source: researchfish
- BBSRC [BB/E003273/1] Funding Source: UKRI
Polarized growth is a fundamental property of cell growth and development. It requires the delivery of post-Golgi secretory vesicles to the site of polarized growth. This process is mediated by Rab GTPases activated by their guanine exchange factors (GEFs). The human fungal pathogen, Candida albicans, can grow in a budded yeast form or in a highly polarized hyphal form, and thus provides a model to study this phenomenon. During hyphal, but not yeast growth, secretory vesicles accumulate in an apical body called a Spitzenkorper, which acts to focus delivery of the vesicles to the tip. Post-Golgi transport of secretory vesicles is mediated by the Rab GTPase Sec4, activated by its GEF Sec2. Using a combination of deletion mapping, in vitro mutagenesis, an analogue-sensitive allele of Cdc28 and an in vitro kinase assay, we show that localization of Sec2 to the Spitzenkorper and normal hyphal development requires phosphorylation of Serine 584 by the cyclin-dependent kinase Cdc28. Thus, as well as controlling passage through the cell cycle, Cdc28 has an important function in controlling polarized secretion. The EMBO Journal (2010) 29, 2930-2942. doi: 10.1038/emboj.2010.158; Published online 16 July 2010
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