期刊
EMBO JOURNAL
卷 30, 期 1, 页码 145-153出版社
WILEY
DOI: 10.1038/emboj.2010.301
关键词
archaea; DNA replication; site-specific recombination; termination; Xer
资金
- Medical Research Council
- Biotechnology and Biological Sciences Research Council, UK
Archaea of the genus Sulfolobus have a single-circular chromosome with three replication origins. All three origins fire in every cell in every cell cycle. Thus, three pairs of replication forks converge and terminate in each replication cycle. Here, we report 2D gel analyses of the replication fork fusion zones located between origins. These indicate that replication termination involves stochastic fork collision. In bacteria, replication termination is linked to chromosome dimer resolution, a process that requires the XerC and D recombinases, FtsK and the chromosomal dif site. Sulfolobus encodes a single-Xer homologue and its deletion gave rise to cells with aberrant DNA contents and increased volumes. Identification of the chromosomal dif site that binds Xer in vivo, and biochemical characterization of Xer/dif recombination revealed that, in contrast to bacteria, dif is located outside the fork fusion zones. Therefore, it appears that replication termination and dimer resolution are temporally and spatially distinct processes in Sulfolobus. The EMBO Journal (2011) 30, 145-153. doi:10.1038/emboj.2010.301; Published online 26 November 2010
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