期刊
EMBO JOURNAL
卷 29, 期 15, 页码 2553-2565出版社
WILEY
DOI: 10.1038/emboj.2010.129
关键词
chromatin; INK4a; Polycomb; transcription; ubiquitination
资金
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BBS/E/B/0000L748, BBS/E/B/0000H234] Funding Source: researchfish
- BBSRC [BBS/E/B/0000H234, BBS/E/B/0000L748] Funding Source: UKRI
An important facet of transcriptional repression by Polycomb repressive complex 1 (PRC1) is the mono-ubiquitination of histone H2A by the combined action of the Posterior sex combs (Psc) and Sex combs extra (Sce) proteins. Here, we report that two ubiquitin-specific proteases, USP7 and USP11, co-purify with human PRC1-type complexes through direct interactions with the Psc orthologues MEL18 and BMI1, and with other PRC1 components. Ablation of either USP7 or USP11 in primary human fibroblasts results in de-repression of the INK4a tumour suppressor accompanied by loss of PRC1 binding at the locus and a senescence-like proliferative arrest. Mechanistically, USP7 and USP11 regulate the ubiquitination status of the Psc and Sce proteins themselves, thereby affecting their turnover and abundance. Our results point to a novel function for USPs in the regulation and function of Polycomb complexes. The EMBO Journal (2010) 29, 2553-2565. doi:10.1038/emboj.2010.129; Published online 2 July 2010
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据