期刊
EMBO JOURNAL
卷 28, 期 22, 页码 3602-3612出版社
WILEY
DOI: 10.1038/emboj.2009.288
关键词
electrophysiology; gating mechanism; ion channel; structural biology
资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Global COE Program (Center of Education and Research for Advanced Genome-Based Medicine)
- Mitsubishi Foundation
The MgtE family of Mg2+ transporters is ubiquitously distributed in all phylogenetic domains. Recent crystal structures of the full-length MgtE and of its cytosolic domain in the presence and absence of Mg2+ suggested a Mg2+-homeostasis mechanism, in which the MgtE cytosolic domain acts as a 'Mg2+ sensor' to regulate the gating of the ion-conducting pore in response to the intracellular Mg2+ concentration. However, complementary functional analyses to confirm the proposed model have been lacking. Moreover, the limited resolution of the full-length structure precluded an unambiguous characterization of these regulatory divalent-cation-binding sites. Here, we showed that MgtE is a highly Mg2+-selective channel gated by Mg2+ and elucidated the Mg2+-dependent gating mechanism of MgtE, using X-ray crystallographic, genetic, biochemical, and electrophysiological analyses. These structural and functional results have clarified the control of Mg2+ homeostasis through cooperative Mg2+ binding to the MgtE cytosolic domain. The EMBO Journal (2009) 28, 3602-3612. doi:10.1038/emboj.2009.288; Published online 1 October 2009
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