期刊
EMBO JOURNAL
卷 28, 期 19, 页码 3052-3063出版社
WILEY-BLACKWELL
DOI: 10.1038/emboj.2009.232
关键词
electron microscopy; Lhc organization; photosystem II
资金
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek through a VIDI
- Partenariats Hubert Curien (Van Gogh project)
Photosystem II ( PSII) is a large multiprotein complex, which catalyses water splitting and plastoquinone reduction necessary to transform sunlight into chemical energy. Detailed functional and structural studies of the complex from higher plants have been hampered by the impossibility to purify it to homogeneity. In this work, homogeneous preparations ranging from a newly identified particle composed by a monomeric core and antenna proteins to the largest C2S2M2 supercomplex were isolated. Characterization by biochemical methods and single particle electron microscopy allowed to relate for the first time the supramolecular organization to the protein content. A projection map of C2S2M2 at 12 angstrom resolution was obtained, which allowed determining the location and the orientation of the antenna proteins. Comparison of the supercomplexes obtained from WT and Lhcb-deficient plants reveals the importance of the individual subunits for the supramolecular organization. The functional implications of these findings are discussed and allow redefining previous suggestions on PSII energy transfer, assembly, photoinhibition, state transition and non-photochemical quenching. The EMBO Journal (2009) 28, 3052-3063. doi: 10.1038/emboj.2009.232; Published online 20 August 2009
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