期刊
EMBO JOURNAL
卷 28, 期 16, 页码 2428-2436出版社
WILEY
DOI: 10.1038/emboj.2009.201
关键词
CTCF; D4Z4; FSHD; nuclear periphery; telomere
资金
- AFM
- Association pour la Recherche contre le Cancer (ARC)
- Programme Emergence de la Region Rhone-Alpes
The localization of genes within the nuclear space is of paramount importance for proper genome functions. However, very little is known on the cis-acting elements determining subnuclear positioning of chromosome segments. We show here that the D4Z4 human subtelomeric repeat localizes a telomere at the nuclear periphery. This perinuclear activity lies within an 80 bp sequence included within a region known to interact with CTCF and A-type Lamins. We further show that a reduced level of either CTCF or A-type Lamins suppresses the perinuclear activities of D4Z4 and that an array of multimerized D4Z4 sequence, which has lost its ability to bind CTCF and A-type Lamins, is not localized at the periphery. Overall, these findings reveal the existence of an 80 bp D4Z4 sequence that is sufficient to position an adjacent telomere to the nuclear periphery in a CTCF and A-type lamins-dependent manner. Strikingly, this sequence includes a 30 bp GA-rich motif, which binds CTCF and is present at several locations in the human genome. The EMBO Journal (2009) 28, 2428-2436. doi:10.1038/emboj.2009.201; Published online 30 July 2009
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