期刊
EMBO JOURNAL
卷 28, 期 18, 页码 2748-2762出版社
WILEY
DOI: 10.1038/emboj.2009.210
关键词
p300; ROS; SENP3; SUMO2/3; ubiquitin-proteasome system
资金
- National Natural Science Foundation of China [30570965]
- Ministry of Science and Technology of China [2006CB910104]
- Shanghai Municipal Science and Technology Commission [08JC1413800, 08ZR1412300]
- Shanghai Municipal Education Commission [J50201]
- NIH [CA-239520, CA-16672]
- McNair
The physiological function of Sentrin/SUMO-specific proteases (SENPs) remains largely unexplored, and little is known about the regulation of SENPs themselves. Here, we show that a modest increase of reactive oxygen species (ROS) regulates SENP3 stability and localization. We found that SENP3 is continuously degraded through the ubiquitin-proteasome pathway under basal condition and that ROS inhibit this degradation. Furthermore, ROS causes SENP3 to redistribute from the nucleoli to the nucleoplasm, allowing it to regulate nuclear events. The stabilization and redistribution of SENP3 correlate with an increase in the transcriptional activity of the hypoxia-inducing factor-1 (HIF-1) under mild oxidative stress. ROS-enhanced HIF-1 transactivation is blocked by SENP3 knockdown. The de-SUMOylating activity of SENP3 is required for ROS-induced increase of HIF-1 transactivation, but the true substrate of SENP3 is the co-activator of HIF-1 alpha, p300, rather than HIF-1 alpha itself. Removing SUMO2/3 from p300 enhances its binding to HIF-1 alpha. In vivo nude mouse xenografts over-expressing SENP3 are more angiogenic. Taken together, our results identify SENP3 as a redox sensor that regulates HIF-1 transcriptional activity under oxidative stress through the de-SUMOylation of p300. The EMBO Journal (2009) 28, 2748-2762. doi: 10.1038/emboj.2009.210; Published online 13 August 2009
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据