期刊
EMBO JOURNAL
卷 28, 期 14, 页码 2042-2052出版社
WILEY
DOI: 10.1038/emboj.2009.121
关键词
mitochondria; pentatricopeptide repeat; plastid; RNA-binding protein; RNA stability
资金
- Deutsche Forschungsgemeinschaft
- USDA-NRI [2006-35318-17380]
- National Science Foundation [DBI-0421799]
Chloroplast mRNA populations are characterized by overlapping transcripts derived by processing from polycistronic precursors. The mechanisms and functional significance of these processing events are poorly understood. We describe a pentatricopeptide repeat (PPR) protein, PPR10, whose binding defines mRNA segments derived from two transcription units in maize chloroplasts. PPR10 interacts in vivo and in vitro with two intergenic RNA regions of similar sequence. The processed 5' and 3' RNA termini in these regions overlap by approximately 25 nucleotides. The PPR10-binding sites map precisely to these overlapping sequences, and PPR10 is required specifically for the accumulation of RNAs with these termini. These findings show that PPR10 serves as a barrier to RNA decay from either the 5' or 3' direction and that a bound protein provides an alternative to an RNA hairpin as a barrier to 3' exonucleases. The results imply that protein 'caps' at both 5' and 3' ends can define the termini of chloroplast mRNA segments. These results, together with recent insights into bacterial RNA decay, suggest a unifying model for the biogenesis of chloroplast transcript populations and for the determinants of chloroplast mRNA stability. The EMBO Journal (2009) 28, 2042-2052. doi: 10.1038/emboj.2009.121; Published online 7 May 2009
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据