期刊
EMBO JOURNAL
卷 28, 期 3, 页码 286-297出版社
WILEY
DOI: 10.1038/emboj.2008.288
关键词
extracellular matrix; innate immunity; integrin; mindin (spondin-2); structure
资金
- National Institutes of Health [AI065612, P01 HL54710, AI054658, AI061364]
- National Synchrotron Light Source
- US Department of Energy
- National Center for Research Resources of the National Institutes of Health
Mindin (spondin-2) is an extracellular matrix protein of unknown structure that is required for efficient T-cell priming by dendritic cells. Additionally, mindin functions as a pattern recognition molecule for initiating innate immune responses. These dual functions are mediated by interactions with integrins and microbial pathogens, respectively. Mindin comprises an N-terminal F-spondin (FS) domain and C-terminal thrombospondin type 1 repeat (TSR). We determined the structure of the FS domain at 1.8-A resolution. The structure revealed an eight-stranded antiparallel beta-sandwich motif resembling that of membrane-targeting C2 domains, including a bound calcium ion. We demonstrated that the FS domain mediates integrin binding and identified the binding site by mutagenesis. The mindin FS domain therefore represents a new integrin ligand. We further showed that mindin recognizes lipopolysaccharide (LPS) through its TSR domain, and obtained evidence that C-mannosylation of the TSR influences LPS binding. Through these dual interactions, the FS and TSR domains of mindin promote activation of both adaptive and innate immune responses.
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