期刊
ELECTROPHORESIS
卷 35, 期 17, 页码 2528-2533出版社
WILEY
DOI: 10.1002/elps.201400083
关键词
MALDI; Microfluidics; Proteomics; SlipChip; Solution IEF
资金
- State key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences [SKLMR-20120604]
- Fundamental Research Funds for the Central Universities
- Renmin University of China [12XNLI04]
- National Natural Sciences Foundation of China [21205134, 31230003]
- National High Technology Research and Development Program of China [2012AA092103]
This paper describes a simple and reusable microfluidic device combining solution IEF (sIEF) with MALDI-TOF MS for rapid proteomic and metabolic analysis of microliter samples. The device contains two glass plates with nanoliter microwell arrays, which can be assembled to form a fluidic path for sIEF separation, and reconfigured for dividing separated bands. One microliter samples can be loaded and separated by sIEF into static bands in 10 approximate to 30 min. After a slipping operation, the static IEF bands can be divided into nanoliter droplets in microwells without mobilization, and the device can be opened for in situ MALDI-TOF MS detection without loss of separation resolution. The performance of the device is characterized by separating and identifying intact proteins. The applicability in metabolic analysis is demonstrated by preliminary experiments on profiling small molecular metabolites in cerebrospinal fluid microdialysates from rat brain.
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