期刊
ELECTROPHORESIS
卷 33, 期 15, 页码 2345-2354出版社
WILEY
DOI: 10.1002/elps.201100646
关键词
Biomarkers; Metabolomics; Nuclear Magnetic Resonance; Urinary profile; Wine intake
资金
- Spanish Ministry of Education and Science [2006-14228-C03-02]
- CONSOLIDER INGENIO [AGL2009-13906-C02-01, AGL 2010-10084-E]
- FUN-C-FOOD from the MICINN, Spain [CSD2007-063]
- MICINN, Spain [ICTS-2009-43]
- Spanish Ministry of Science and Innovation (MICINN)
- European Social Funds
- Leonardo da Vinci Programme
- European Commission
Moderate wine consumption is associated with health-promoting activities. An H-NMR-based metabolomic approach was used to identify urinary metabolomic differences of moderate wine intake in the setting of a prospective, randomized, crossover, and controlled trial. Sixty-one male volunteers with high cardiovascular risk factors followed three dietary interventions (28 days): dealcoholized red wine (RWD) (272mL/day, polyphenol control), alcoholized red wine (RWA) (272mL/day) and gin (GIN) (100mL/day, alcohol control). After each period, 24-h urine samples were collected and analyzed by 1H-NMR. According to the results of a one-way ANOVA, significant markers were grouped in four categories: alcohol-related markers (ethanol); gin-related markers; wine-related markers; and gut microbiota markers (hippurate and 4-hydroxphenylacetic acid). Wine metabolites were classified into two groups; first, metabolites of food metabolome: tartrate (RWA and RWD), ethanol, and mannitol (RWA); and second, biomarkers that relates to endogenous modifications after wine consumption, comprising branched-chain amino acid (BCAA) metabolite (3-methyl-oxovalerate). Additionally, a possible interaction between alcohol and gut-related biomarkers has been identified. To our knowledge, this is the first time that this approach has been applied in a nutritional intervention with red wine. The results show the capacity of this approach to obtain a comprehensive metabolome picture including food metabolome and endogenous biomarkers of moderate wine intake.
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