期刊
ELECTROPHORESIS
卷 32, 期 23, 页码 3399-3405出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.201100078
关键词
DNA extraction; Droplet processing; Droplet splitting; Reagent addition
资金
- 973 Program [2007CB714505, 2007CB714507]
- CAS [KJCX2-YW-H18, YZ200908]
This work describes a novel droplet-based microfluidic device, which enables sequential droplet processing for rapid DNA extraction. The microdevice consists of a droplet generation unit, two reagent addition units and three droplet splitting units. The loading/washing/elution steps required for DNA extraction were carried out by sequential microfluidic droplet processing. The movement of superparamagnetic beads, which were used as extraction supports, was controlled with magnetic field. The microdevice could generate about 100 droplets per min, and it took about 1?min for each droplet to perform the whole extraction process. The extraction efficiency was measured to be 46% for ?-DNA, and the extracted DNA could be used in subsequent genetic analysis such as PCR, demonstrating the potential of the device for fast DNA extraction.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据