期刊
ELECTROPHORESIS
卷 32, 期 22, 页码 3133-3137出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.201100161
关键词
Embryonic stem cell; Endothelial cell differentiation; Integrated microfluidic device
资金
- Ministry of Health & Welfare, Republic of Korea [A100195]
- Korea Health Promotion Institute [A100195] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We developed an integrated microfluidic culture device to regulate embryonic stem (ES) cell fate. The integrated microfluidic culture device consists of an air control channel and a fluidic channel with 4 x 4 micropillar arrays. We hypothesized that the microscale posts within the micropillar arrays would enable the control of uniform cell docking and shear stress profiles. We demonstrated that ES cells cultured for 6 days in the integrated microfluidic culture device differentiated into endothelial cells. Therefore, our integrated microfluidic culture device is a potentially powerful tool for directing ES cell fate.
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