期刊
ELECTROPHORESIS
卷 32, 期 8, 页码 884-889出版社
WILEY
DOI: 10.1002/elps.201000420
关键词
CE; Contactless conductivity detection; Lactate; Lactate threshold; Plasma lactate
资金
- Thai Government
- Swiss National Science Foundation [200020-126384/1]
- Swiss National Science Foundation (SNF) [200020_126384] Funding Source: Swiss National Science Foundation (SNF)
The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2-morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL-histidine, 70 mu mol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10-1000 mu mol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 mu mol/L. Intra-and inter-day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.
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