期刊
ELECTROPHORESIS
卷 31, 期 19, 页码 3342-3345出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.201000310
关键词
Capillary electrophoresis; Chemiluminescence; Diperiodatocuprate(III); Luminol; N-(4-Aminobutyl)-N-ethylisoluminol
资金
- Natural Science Foundation of Chongqing (CSTC) [2009BB5116]
- Fundamental Research Funds for the Central Universities [XDJK2009B014]
Luminol-type compounds can be used as chemiluminescent (CL) derivatization reagents for amines, carboxylic acids and protein. Copper chelate diperiodatocuprate(III) (K-5[Cu(HIO6)(2)], DPC) was synthesized by complexation of copper at trivalent oxidation state and periodate in a strong basic medium. It was found that DPC can greatly enhance the reaction between luminol-type compounds and H2O2 to produce very strong CL emission. Based on this fact, a rapid CE method combined with high-sensitive end-column CL detection was established to simultaneously analyze luminol and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) with wide concentration range of 3.0-300 nmol/L in 5 min. The RSDs of the signal intensity and the migration time were less than 3.9 and 7.0% for a standard sample containing 100 nmol/L luminol and ABEI (n = 5), respectively. The investigation implies that DPC is a promising sensitizer for CE-CL detection of a great variety of biomolecules and drugs in biological samples after derivatization using luminol derivatives.
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