期刊
ELECTROPHORESIS
卷 31, 期 3, 页码 570-574出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elps.200900404
关键词
CE; Contactless conductivity detection; Drug analysis; Electrochemical detection; Tuberculosis
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [04/09079-0, 06/07309-3]
- Conselho Nacional de Pesquisa e Desenvolvimento (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [06/07309-3] Funding Source: FAPESP
A method for the determination of ethambutol (EMB), a first-line drug against tuberculosis, based on CE with capacitively coupled contactless conductivity detection is proposed. The separation of EMB and its main product of degradation were achieved in less than 3 min with a resolution of 2.0 using a BGE composed of 50 mmol/L histidine and 30 mmol/L MES, pH 6.30. By raising the pH to 8.03, the analysis time was reduced to 1.0 min, but with a significant loss of resolution (0.7). Using the best separation 2 conditions, linearity of 0.9976 (R(2) five data points), sensitivity of 1.26 x 10(-4) V min mu mol(-1) L, and LOD and quantification of 23.5 and 78.3 mu mol/L, respectively, were obtained. Recoveries at four levels of concentration ranged from 95 to 102% and the concentration range studied ranged from 100 to 500 mu mol/L. The results obtained for the determination of EMB in pharmaceutical formulations were compared with those obtained by using CE with photometric detection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据