期刊
ELECTROPHORESIS
卷 30, 期 9, 页码 1497-1500出版社
WILEY
DOI: 10.1002/elps.200800563
关键词
Bioassay; Microfluidics; Paper-based microfluidics; Wax
资金
- National Nature Science Foundation of China [20575067, 20635030, 90713014]
- Ministry of Science and Technology of China [2006AA02Z305, 2006AA020201, 2007CB714505, 2007CB714507]
- National Science & Technology Pillar Program in the Eleventh Five-year Plan Period of China [2006-BAD12B03-01]
- Innovation Project of Chinese Academy of Sciences
Here we present a simple and low-cost production method to generate paper-based microfluidic devices with wax for portable bioassay. The wax patterning method we introduced here included three different ways: (i) painting with a wax pen, (ii) printing with an inkjet printer followed by painting with a wax pen, (iii) printing by a wax printer directly. The whole process was easy to operate and could be finished within 5-10 min without the use of a clean room, UV lamp, organic solvent, etc. Horse radish peroxidase, BSA and glucose assays were conducted to verify the performance of wax-patterned paper.
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