期刊
ELECTROPHORESIS
卷 30, 期 4, 页码 661-667出版社
WILEY
DOI: 10.1002/elps.200800555
关键词
ACN; Field-amplified sample injection; Isoquinoline alkaloids; Urine MEEKC
资金
- National Natural Science Foundation of China [20735002, 20575011, 20877019]
- Key Natural Sciences Foundation of Fujian Province, China [D0520001]
New methods based on MEEKC coupling with field-amplified sample injection (FASI) induced by ACN were proposed for five isoquinoline alkaloids (berberine, palmatine, jatrorrhizine, sinomenine and homoharringtonine) in no salt and high salt sample solution (HS). For the separation of five isoquinoline alkaloids, a running buffer composed of 18 mM sodium cholate, 2.4%v/v butan-1-ol, 0.6%v/v ethyl acetate, 10%v/v (or 30% v/v) methanol and 87.0% v/v (or 67% v/v) 5 mM Na2B4O7 similar to 10 mM NaH2PO4 buffer (pH 7.5) was developed. in order to improve the sensitivity, FASI induced by ACN was applied to increase the detection sensitivity. The detection limit was found to be as low as 0.0002 mu g/mL in no salt sample solution and 0.062 mu g/mL in HS. The method has been applied for the analysis of human urine spiked with analytes, and the assay results were proved to be satisfactory, and also the determination of berberine in urine sample after oral administration berberine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据