期刊
ELECTROPHORESIS
卷 29, 期 10, 页码 2101-2111出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.200700465
关键词
8-Aminopyrene-1,3,6-trisulfonate; galacturonic acid; mass spectrometry; oligomers; pectin
As pectin molecules are too large and heterogeneous to analyze as a whole, the polymer is usually degraded to smaller oligomers, which are often analyzed by high-performance anion exchange chromatography (HPAEC). However, the high salt concentration necessary to elute pectin oligomers by HPAEC is incompatible with online mass detection. To overcome such a disadvantage, a CE-IT-MS system was set up to further elucidate the fine structure of charged oligosaccharides. An effective separation of differently substituted galacturonic acid containing oligomers was obtained by low-pH CE-LIF analysis. By adapting the buffer and capillary online MS detection was enabled. Moreover, with MS/MS it was possible to localize sugar residues' substitutions. With this combined CE-MS approach LIF electropherograms of xylogalacturonan and rhamnogalacturonan I digests could be annotated. The method was further exemplified by a complex oligomer mixture of acid hydrolyzed apple pectin, which was separated and characterized by CE-MS(n). Oligomers present in low amounts could be localized by their corresponding m/z, as was demonstrated by selected mass range representation.
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