期刊
ELECTROPHORESIS
卷 29, 期 24, 页码 5033-5046出版社
WILEY
DOI: 10.1002/elps.200800166
关键词
ABO blood type; Dielectrophoresis; Erythrocytes; Medical diagnostics; Microdevice
资金
- NSF CAREER [0644538]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0644538] Funding Source: National Science Foundation
Dielectrophoretic manipulation of erythrocytes/red blood cells is investigated as a tool to identify blood type for medical diagnostic applications. Positive blood types of the ABO typing system (A+, B+, AB+ and O+) were tested and cell responses quantified. The dielectrophoretic response of each blood type was observed in a platinum electrode microdevice, delivering a field of 0.025V(pp)/mu m at 1 MHz. Responses were recorded via video microscopy for 120 s and erythrocyte positions were tabulated at 20-30 s intervals. Both vertical and horizontal motions of erythrocytes were quantified via image object recognition, object tracking in MATLAB, binning into appropriate electric field contoured regions (wedges) and statistical analysis. Cells of O+ type showed relatively attenuated response to the dielectrophoretic field and were distinguished with greater than 95% confidence from all the other three blood types. AB+ cell responses differed from A+ and B+ blood types likely because AB+ erythrocytes express both the A and B glycoforms on their membrane. This research suggests that dielectrophoresis of untreated erythrocytes beyond simple dilution depends oil blood type and could be used in portable blood typing devices.
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