期刊
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
卷 17, 期 1, 页码 -出版社
UNIV CATOLICA DE VALPARAISO
DOI: 10.1016/j.ejbt.2013.12.006
关键词
Biosurfactant; Candida lipolytica; Lipopeptide; Surface tension; Toxicity
资金
- Foundation for the Support of Science and Technology of the State of Pernambuco (FACEPE)
- Thermoelectric of Pernambuco (TERMOPE)
- National Agency of Electric Energy (ANEEL)
- National Council for Scientific and Technological Development (CNPq)
- Coordination for the Improvement of Higher Level Education Personnel (CAPES)
Background: Biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have been established. Results: The yeast was grown for 72 h to determine the kinetics of growth and production. The surface tension of the cell-free broth was reduced from 55 to 25 mN/m. The yield of biosurfactant was 8.0 g/l with a CMC of 0.03%. The biosurfactant was characterized as an anionic lipopeptide composed of 50% protein, 20% lipids, and 8% of carbohydrates. Conclusions: The isolated biosurfactant showed no toxicity against different vegetable seeds: Brassica oleracea, Solanum gilo and Lactuca sativa L. and the micro-crustacean Artemia salina. The properties of the biosurfactant produced suggest its potential application in industries that require the use of effective compounds at low cost. (C) 2014 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B. V. All rights reserved.
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