期刊
ELECTROCHIMICA ACTA
卷 140, 期 -, 页码 550-556出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2014.05.065
关键词
biofunctionalization; cytochrome c; porous silicon; bioelectrode; cyclic voltammetry
资金
- CONACYT through the Mexico-Hungary bilateral program
- Ciencia Basica [193555, 153161]
- CONACYT [24783]
- UJAT-(PISA)
Quasi-reversible and direct electrochemistry has been observed at a new electrochemical interface constructed by functionalization of porous silicon microcavities (PSiMc) with either amino (APTES) or mercapto (MPTS) propyltriethoxysilane and the redox protein cytochrome c (cyt c). The high specific surface area, tunable pore size (observed by scanning electron microscopy) and functional surface of PSiMc permitted the infiltration of molecular binders and cyt c into the porous structure. The optical response of PSiMc was evaluated during all modification steps. In all the cases a red shift in the reflectance spectra after substrate modification was observed, indicating the infiltration and immobilization of organic binders and the cyt c protein. Dose-response curves demonstrated that biofunctionalization with APTES followed by glutaraldehyde (GTA) amine activation provides higher amounts of immobilized protein with 4.73 nmol protein/cm(2) of etching area compared with 1 nmol protein/cm(2) when using MPTS as a molecular binder. Infrared spectroscopy (FTIR) also demonstrated the adsorption of the protein within the surface nanostructure. The PSiMc/cyt c hybrid devices showed high electrochemical stability due to the retaining of the protein's redox activity even after several months of its immobilization (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据