期刊
ELECTROCHIMICA ACTA
卷 89, 期 -, 页码 13-17出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2012.10.155
关键词
Magnetic graphene oxide; Electrochemiluminescence (ECL); Thrombin; Aptamer; Iridium(III) complex
资金
- National Basic Research Program of China [2010CB732403]
- NSFC [211710384, 41076059]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
A new electrochemiluminescence (ECL) reagent, iridium(III) complex (Kill) complex), has been synthesized and applied to develop an ECL biosensor for thrombin. The Ir(III) complex has been conjugated to aptamers firstly and adsorbed on magneto-controlled magnetic graphene oxide. In the presence of the target (thrombin), the aptamers will release into solution and result in increased ECL intensity. The enhanced ECL intensity has a direct relationship with the logarithm of thrombin concentration in the range of 2.0 x 10(-9) to 5.0 x 10(-8) mol/L. The detection limit was 1.3 x 10(-9) mol/L (S/N = 3). The proposed method has good stability and reproducibility. (C) 2012 Elsevier Ltd. All rights reserved.
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