期刊
ELECTROCHIMICA ACTA
卷 56, 期 24, 页码 8168-8175出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2011.05.128
关键词
Electrochemical immunosensor; Silver nanowire; Graphene nanosheet; Biomarker; Alpha-fetoprotein
资金
- National Natural Science Foundation of China [21075019, 20735002]
- Research Fund for the Doctoral Program of Higher Education of China [20103514120003]
- National Basic Research Program of China [2010CB732403]
- Program for Returned High-Level Overseas Chinese Scholars of Fujian Province [XRC-0929]
A facile and sensitive mediator-free electrochemical immunoassay was developed for determination of alpha-fetoprotein (AFP) in human serum with a sandwich-type mode by using silver nanowire-graphene hybrid nanocomposites (AgNW-GPs) as label. One-pot hydrothermal method was used for the synthesis of the AgNW-GPs with the aid of trisodium citrate. The as-prepared AgNW-GP was not only utilized for the label of horseradish peroxidase-conjugated anti-AFP (HRP-anti-AFP) with highly loading capacity, but also displayed good electrochemical behaviors. Compared with the conventional HRP-anti-AFP-based electrochemical immunoassay, the sensitivity of the proposed immunoassay by using the synthesized nanolabels was greatly improved. Under optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.05-400 ng/mL with a low detection limit (LOD) of 5 pg/mL AFP (at 3s(B)). Intra- and inter-assay coefficients of variation were below 10% and 8.5%, respectively. The selectivity and stability of the immunosensor were acceptable. In addition, the methodology was also validated by assaying 16 positive serum samples and 7 negative serum samples, receiving a good correlation with the results obtained from the referenced electrochemiluminescence method. (C) 2011 Elsevier Ltd. All rights reserved.
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