期刊
ELECTROCHIMICA ACTA
卷 55, 期 24, 页码 7385-7393出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2010.07.026
关键词
Laccase; Bilirubin oxidase; Ascorbate oxidase; Bio-cathode; Enzymatic fuel cell
资金
- Bio/Nano Architectures (UNM) [MR 2D100]
Multicopper oxidases (MCO) have been extensively studied as oxygen reduction catalysts for cathodic reactions in biofuel cells Theoretically. direct electron transfer between an enzyme and electrode offers optimal energy conversion efficiency providing that the enzyme/electrode interface can be engineered to establish efficient electrical communication. In this study, the direct bioelectrocatalysts of three MCO (Laccase from Trametes versicolor, bilirubin oxidase (BOD) from the fungi Myrothecium verrucaria and ascorbate oxidase(AOx) from Cucurbita sp.) was investigated and compared as oxygen reduction Protein film voltammetry and electrochemical characterization of the MCO electrodes showed that DET had been successfully established in all cases. Atomic force microscopy imaging and force measurements indicated that enzyme was immobilized as a monolayer on the electrode surface Evidence for three clearly separated anodic and cathodic redox events related to the Type 1 (T1) and the trinculear copper centers (T2, T3) of various MCO was observed. The redox potential of the T1 center was strongly modulated by physiological factors including pH, anaerobic and aerobic conditions and the presence of inhibitor S. (C) 2010 Elsevier Ltd All rights reserved.
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