A novel electrochemical immunosensor for Golgi Protein 73 assay

In this work, we have developed an electrochemical immunosensor for the assay of Golgi Protein 73 (GP73), which is a newly-established and highly sensitive biomarker for biliary tract cancer. Intrinsic specificity and sensitivity of this immunosensor can be achieved due to the good conductivity of electrochemically reduced graphene oxide, and the prominent electrochemical signal of quantum dots. So, our sensor can enable a limit of detection as low as 12 pM, and it can make direct serum assay possible, the results of which may clearly predict the effect of surgical removal of the primary tumor, indicating potential clinical application of our sensor in the future. (c) 2014 Elsevier B.V. All rights reserved.