期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 33, 期 -, 页码 55-58出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2013.04.009
关键词
MicroRNA; Cancer diagnostics; Electrochemistry; Mercury electrodes
资金
- RECAMO [CZ.1.05/2.1.00/03.0101]
- GACR [P206/12/G151, P301/11/2055]
Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and 2,2'-bipyridine, Os(VI)bipy, specifically binding to the 3'-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labeling step with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture of other noncomplementary miRNAs was possible. (c) 2013 Elsevier B.V. All rights reserved.
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