Regulatory peptides desmopressin and glutathione voltammetric determination on nickel oxide modified electrodes

The determination of important regulatory peptides containing disulphidic bridge and thiols are of much interest. We investigated, via differential pulse voltammetry, the use of glassy carbon electrodes modified with nickel(II) oxide nanoparticles for electrocatalytic oxidation of L-glutathione and desmopressin. Linear response in the range of 0.2 to 6.0 mM of L-glutathione was observed, which brackets the concentration of about 5 mM of L-glutathione in living cells. Hence electrochemical detection of L-glutathione can be achieved using the simple preparation of GC electrodes modified with inexpensive NiO nanoparticles. (C) 2011 Elsevier B.V. All rights reserved.