期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 12, 期 2, 页码 258-261出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2009.12.008
关键词
Aptamer; Microchip; Thrombin; Electrochemistry; Sensor
资金
- National Natural Science Foundation of China [20775016]
- 973 Program [2007CB714506]
- China Postdoctoral Science Foundation [20070420084]
- Shanghai Postdoctoral Science Foundation [07R214105]
In this paper, a microchip-based sandwich-type aptasensor is developed for the detection of human thrombin. The SH-aptamer/thrombin/alkaline phosphatase-functionalized aptamer (ALP-aptamer) system was constructed in the microfluidic channels. And the substrate solution containing 4-aminophenyl phosphate (p-APP) was introduced to the microchannels for the end-column electrochemical detection. The on-chip aptasensor has a broad linear response range of 1-100 pM with a detection limit of 1 pM, which shows high sensitivity and specificity. The system was then applied to detect thrombin in human serum sample. Therefore, the on-chip aptasensor has a great promise for detecting and screening ultra-trace levels of biomarkers in the complex matrices. (C) 2009 Elsevier B.V. All rights reserved.
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