Synthesis, characterizations of silica-coated gold nanorods and its applications in electroanalysis of hemoglobin

Gold nanorods (GNRs) were synthesized by a seed-mediated growth approach followed by TEOS polymerization leading to the formation of silica layer surrounding the gold nanorod core. TEM images showed that the silica-coated gold nanorods (GNRs@SiO2) were dispersed with an average aspect ratio of 3.1 for the GNRs cores and a uniform thickness of the silica shell. The core/shell nanocomposites were further used as efficient supports for the immobilization of hemoglobin (Hb) to fabricate a novel biosensor. The immobilized Hb showed an enhanced electron transfer for its heme Fe(III) to Fe(II) redox couple. This biosensor showed an excellent bioelectrocatalytic activity towards H2O2 with a linear range from 8.0 x 10(-7) to 6.1 x 10(-5) M, and the detection limit was 6.0 x 10(-8) M at 3 sigma. The apparent Michaelis-Menten constant of the immobilized hemoglobin was calculated to be 0.13 mM. (C) 2008 Elsevier B.V. All rights reserved.