期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 10, 期 3, 页码 492-495出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2008.01.013
关键词
chitosan; TiO2; antibodies; ochratoxin A; immunosensor
Fourier transform infrared (FT-IR) and UV-visible spectroscopy were used to optimize TiO2 concentration in chitosan (CS) to develop a sensitive CS/TiO2 bioactive electrode. Electrochemical impedance spectroscopy (EIS) used to measure electro-activity of these bioactive electrodes associated with enhance oligosaccharide containing -C=O groups from degradation of CS molecules. This matrix has free -NH2 and -OH functional groups due to higher probability of hydrogen and covalent bonding between -OH group in CS molecules with Ti-O-Ti which supported immobilization of rabbit antibodies (IgGs) and proteins. Ochratoxin A (OTA) was detected and showed a linear response up to 10 ng/mL with CS/TiO2 bio-electrode. The OTA detection sensitivity of 7.5 mM TiO2 added CS bioactive electrode was four times higher than only CS. (c) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据