期刊
ELECTROCHEMISTRY
卷 76, 期 8, 页码 555-558出版社
ELECTROCHEMICAL SOC JAPAN
DOI: 10.5796/electrochemistry.76.555
关键词
cell adhesion; dielectrophoresis; elelctrochemical biolithography; microfluidic device
We describe a method to create cell-adhesive regions and to position adherent cells on the newly created regions in sequence within a microfluidic channel. One of the microelectrodes fabricated at the channel wall was used for locally electrogenerating hypobromous acid that renders the opposite face of the channel protein-adsorptive. After the fibronectin was immobilized on the treated region, an ac voltage (1 MHz, 20 Vpp) was applied to the microelectrodes array in the presence of suspended HeLa cells. Since a repulsive force of negative dielectrophoresis (DEP) directs the cells toward the weakest region of the nonuniform electric field, the cells were positioned on the fibronectin-patterned region to allow the cell adhesion, even in the presence of fluid flow (< 0.1 mu L min(-1)). By repeating the above process, two types of cells could be patterned in the microchannel.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据