期刊
ELECTROANALYSIS
卷 30, 期 10, 页码 2374-2385出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201800360
关键词
Uricase; enzymatic biosensor; cationic polymer; wound monitoring; non-invasive detection
资金
- NSF Nanosystems Engineering Research Centre for Advanced Self-Powered Systems of Integrated Biosensors and Technologies (AS-SIST) [EEC-1160483]
In this work, an electrochemical approach using uricase (UOx) as an enzyme to detect uric acid (UA) for wound monitoring has been reported. UOx was entrapped in poly (vinyl alcohol) N-methyl-4(4'-formylstyryl) pyridinium methosulfate acetal (PVA-SbQ), a cationic polymer matrix. The polymer-enzyme ratio for immobilization was calculated as 53.2g cm(-2):0.25Ucm(-2). UA was detected both optically as well as electrochemically. A redox electron shuttle, ferrocene carboxylic acid (FCA) was used to facilitate electron transfer. Entrapped UOx provided improved response to UA detection compared to physisorbed UOx. Sensor response was linear in the physiologically relevant ranges between 12 and 100M. The entrapped UOx biosensor was stable for 48h and maintained 90% activity until 5days. This entrapped biosensor was used for UA measurements in biofluids of sweat and wounds. The sensor demonstrated a recovery of approximate to 102-107%. These results show that entrapment of UA in such a polymer matrix is a preferred approach for UA measurements under physiological conditions.
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