Spontaneous Deposition of Prussian Blue on Reduced Graphene Oxide - Gold Nanoparticles Composites for the Fabrication of Electrochemical Biosensors

A reduced graphene oxide-gold nanoparticles-Prussian blue (RGO-AuNPs-PB) composite was prepared by spontaneous deposition of PB on RGO-AuNPs surface. The GC/RGO-AuNPs-PB modified electrode offered good electrocatalytic activity toward the reduction of hydrogen peroxide, indicating the possible synergistic effects of the RGO-AuNPs-PB composite material. Direct electron transfer of glucose oxidase (GOD) can be realized after codeposition of GOD and chitosan (CHIT) coating on GC/RGO-AuNPs-PB electrode. The resulting GC/RGO-AuNPs-PB/GOD-CHIT biosensor exhibited an excellent amperometric response to glucose with a sensitivity of 84 mA M-1 cm(-2), and a linear range from 13 mu M to 2.0 mM at + 0.1 V vs. Ag vertical bar AgCl reference.