Renewable Pencil Electrodes for Highly Sensitive Anodic Stripping Voltammetric Determination of 3-Hydroxyflavone and Morin in Bulk Form and in Biological Fluids

An electrochemical anodic adsorptive stripping procedure for ultra-trace assay of 3-hydroxyflavone (3HF) and Morin at a renewable pencil electrode (PGE) in bulk form and in biological fluids is described. The nature of the oxidation process of 3HF and Morin taking place at the PGE was characterized by cyclic voltammetry. The results show that the determination of the oxidation peak current is the basis of a simple, accurate and rapid method for quantification of 3HF by square-wave anodic stripping voltammetry. Determination of Morin was achieved by square-wave anodic adsorptive stripping voltammetry of the formed MorinCu(II) complex at a PGE. Factors influencing the trace measurements of 3HF and the MorinCu (II) complex at a PGE are assessed. The limits of detection and quantitation for the determination of 3HF and Morin in bulk form and in biological fluids were determined. The statistical analysis and the calibration curve data for trace determination of 3HF and Morin are reported.