期刊
ELECTROANALYSIS
卷 25, 期 2, 页码 468-474出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201200475
关键词
Biosensors; pH-FET; Urease; Zeolites
资金
- European Project IRSES-NANODEVICE
- National Academy of Sciences of Ukraine
Different modifications of the zeolites Na+-Beta and LTA were applied for improving the working characteristics of a urea biosensor. The bioselective membrane of the biosensor was based on urease and different zeolites co-immobilized with bovine serum albumin on the surface of a pH-FET. It was shown that the biosensors modified with the zeolites H+-Beta30 and H+-Beta50 are characterized by increased sensitivity to urea. The influence of the zeolite concentration on the sensitivity of the biosensors was studied. The optimal concentration of the zeolites H+-Beta30 and H+-Beta50 in the bioselective membrane was 15%. Different variants of co-immobilization of urease and zeolite H+-Beta30 were studied and the optimal method was selected. Thus, a general conclusion is that the urea biosensor sensitivity can be improved using zeolite H+-Beta30 for urease immobilization in the bioselective membrane.
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