期刊
ELECTROANALYSIS
卷 25, 期 8, 页码 1887-1894出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201300071
关键词
Ion-selective electrodes; Potentiometry; Electrochemical biosensors; Polyaniline; Protein adsorption; Conducting polymers
A potentiometric sensor for studying charge based adsorption of proteins was created using a single-piece polyaniline-PVC ion-selective electrode (ISE). Three different ISEs, two for Na+ and one for Cl- ion determination, were studied. The Na+-ISEs consisted of a neutral calixarene-based ionophore and one with a charged carrier dinonylnapthalenesulfonic acid (DNNSA) whereas for the Cl- ISE, an anion exchanger tridodecylmethylammonium chloride (TDDMA(+)Cl(-)), was used. The Na+ ISE with DNNSA as the charged carrier was successfully able to discriminate the binding of two different proteins (bovine serum albumin and lysozyme) based on their intrinsic charge.
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