期刊
ELECTROANALYSIS
卷 23, 期 6, 页码 1418-1426出版社
WILEY-BLACKWELL
DOI: 10.1002/elan.201000611
关键词
Electrochemiluminescence (ECL); Ru(bpy)(3)(2+); Ru-PtNPs; Electroactive surface area; alpha-Fetoprotein antigen (AFP)
资金
- NNSF of China [21075100]
- Ministry of Education of China [708073]
- Natural Science Foundation of Chongqing City [CSTC-2009BA1003]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC 2010009]
- Southwest China University [KB 2010006]
- High Technology Project Foundation of Southwest University, China [XSGX02]
A approach was successfully employed for constructing a solid-state electrochemiluminescence (ECL) immunosensor by layer-by-layer self-assembly of multiwall carbon nanotubes (MWCNTs)-Nafion composite film, Ru(bpy)(3)(2+)/nano-Pt aggregates (Ru-PtNPs) and Pt nanoparticles (PtNPs). The influence of Pt nanoparticles on the ECL intensity was quantitatively evaluated by calculating the electroactive surface area of different electrodes with or without PtNPs to immobilize Ru(bpy)(3)(2+). The principle of ECL detection for target alpha-fetoprotein antigen (AFP) was based on the increment of resistance after immunoreaction, which led to a decrease in ECL intensity. The linear response range was 0.01-10 ngmL(-1) with the detection limit of 3.3 pg mL(-1). The immunosensor exhibited advantages of simple preparation and operation, high sensitivity and good selectivity.
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