期刊
ELECTROANALYSIS
卷 23, 期 6, 页码 1383-1390出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000753
关键词
Calf thymus DNA; 2,8-Dihydroxyadenine; Edge plane pyrolytic graphite electrode; Single-wall carbon nanotubes; Square wave voltammetry
资金
- Council of Scientific and Industrial Research, New Delhi
- Department of Science and Technology, New Delhi
A novel and reliable direct electrochemical method has been established to monitor DNA damage in acid hydrolyzed calf thymus DNA, based on the determination of 2,8-dihydroxyadenine (2,8-DHA). A single-wall carbon nanotubes (SWCNT) modified edge plane pyrolytic graphite electrode (EPPGE) has been used as a sensor to monitor the DNA damage. 2,8-DHA the main in vivo adenine oxidation product undergoes oxidation at similar to 395 mV at SWCNT modified EPPGE using square wave voltammetry (SWV). The sensor exhibits potent and persistent electron-mediating behavior. A well-defined oxidation peak for the oxidation of 2,8-DHA was observed at modified electrode with lowering of peak potential and increase in peak current as compared to bare EPPGE. At optimal experimental conditions, the catalytic oxidative peak current was responsive with the 2,8-DHA concentrations ranging from 0.05 nM to 100 nM. The detection limit was 3.8 x 10(-11) M and limit of quantification was 1.27 x 10(-10) M. The modified electrode exhibited high stability and reproducibility.
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