Facile Synthesis of Leaf-Like CuO Nanoparticles and Their Application on Glucose Biosensor

An efficient amperometric biosensor based on well-crystallized leaf-like CuO nanoparticles for detecting glucose has been proposed. The leaf-like CuO nanoparticles, synthesized by a simple one-step hydrothermal method, were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscopy (TEM) for the morphology study. Under the optimal condition, the electrochemical behaviour of the leaf-like CuO nanoparticles modified electrode for detection of glucose exhibited high sensitivity of 246 mu A/mM/cm(2), short response time (within 5 s), linear dynamic range from 1.0 to 170 mu M (R(2) = 0.9995), and low limit of detection (LOD) (S/N= 3) of 0.91 mu M. The high sensitivity, good reproducibility, stability, and fast amperometric sensing towards oxidation of glucose, make this biosensor promising for future application.