期刊
ELECTROANALYSIS
卷 22, 期 22, 页码 2665-2671出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000285
关键词
DNA interaction; Gatifloxacin; Moxifloxacin; Sparfloxacin; Voltammetry
The electrochemical oxidation of the three fluoroquinolone drugs FQs: gatifloxacin GTF, moxifloxacin MXF and sparfloxacin SPF, at the bare and DNA-modified glassy carbon electrodes has been studied by voltammetric techniques. The three FQs showed one irreversible oxidation peak at potential range 0.85-0.91 V vs. Ag-AgCl, in phosphate buffer of pH 7.0. Differential pulse voltammetry (DPV) and UV-absorption spectroscopic techniques were employed to probe the interaction between the FQs and calf thymus double stranded deoxyribonucleic acid (ds CT-DNA). From electrochemical data, the binding constant between DNA and the gatifloxacin, moxifloxacin and sparfloxacin are calculated to be 3228, 2596 and 2857 M-1 respectively. Based on electrochemical and spectroscopic results, the mode of binding of fluoroquinolone to DNA through combined effect of intercalation and electrostatic interaction was concluded. A detection scheme based on a preconcentration and differential pulse voltammetric (DPV) determination at dsDNA modified glassy carbon electrode (DNA/GCE) was proposed for the trace determination of the studied analytes. The developed method was successfully applied to the determination of the FQs in pharmaceutical formulations.
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