CuO-Doped Mesoporous Silica Hybrid for Rapid and Sensitive Amperometric Detection of Phenolic Compounds

This work constructed an amperometric biosensing platform using CuO doped mesoporous silica hybrid (CuO/SBA-15) as a carrier. The CuO/SBA-15 showed a pair of redox peaks of Cu2+/0. Upon immobilization of tyrosinase on the hybrid, the resulting biosensor exhibited a rapid (<0.5 s) and sensitive amperometric response to phenolic compounds under the optimized conditions. The linear response to catechol ranged from 1.2 x 10(-9) to 3.0 x 10(-5) M. The activation energy for enzymatic reaction was calculated to be 26.6 kJ mol(-1). The apparent Michaelis-Menten constants of the enzyme electrode were estimated to be 54.6, 145, 17.0, 74.8 and 633 mu M for catechol, phenol, p-cresol, m-cresol and dopamine hydrochloride, respectively. The metal oxide doped mesoporous silica hybrid exhibited excellent performance for construction of new biosensors.