期刊
ELECTROANALYSIS
卷 22, 期 3, 页码 259-264出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200900321
关键词
Glucose biosensor; Graphene; Au nanoparticles; Electrocatalytic oxidation; Synergistic effect; Biosensors; Nanoparticles
资金
- National Natural Science Foundation of China [20676038]
- Key Project of Science and Technology for Ministry of Eductaion [107045]
- Innovation Program of Shanghai Municipal Education Commission [09ZZ58]
- Program of Shanghai Subject Chief Scientist [OSXDI.401500]
- Shuguang ScholarTracking Foundation of Shanghai [08GG09]
- Shanghai Leading Academic Discipline Project [B502]
Graphene was successfully prepared and well separated to individual sheets by introducing -SO3-. XRD and TEM were employed to characterize the graphene. UV-visible absorption spectra indicated that glucose oxidase (GOx) could keep bioactivity well in the grapene-Au biocomposite. To construct a novel glucose biosensor, graphene, Au and GOx were co-immobilized in Nafion to further modify a glassy carbon electrode (GCE). Electrochemical measurements were carried out to investigate the catalytic performance of the proposed biosensor. Cyclic voltammograms (CV) showed the biosensor had a typical catalytic oxidation response to glucose. At the applied potential +0.4 V, the biosensor responded rapidly upon the addition of glucose and reached the steady state current in 5 s, with the present of hydroquinone. The linear range is from 15 mu M to 5.8 mM, with a detection limit 5 mu M (based oil the S/N=3). The Michaelis-Menten constant was calculated to be 4.4 mM according to Lineweaver-Burk equation. In addition, the biosensor exhibits good reproducibility and long-term stability. Such impressive properties could be ascribed to the synergistic effect of graphenc-Au integration and good biocompatibility of the hybrid material.
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