期刊
ELECTROANALYSIS
卷 22, 期 16, 页码 1817-1823出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000197
关键词
Nanoparticles; Biosensors; Glucose; Enzyme electrode; Electropolymerization; Reconstitution
资金
- Israel Science Foundation
Au nanoparticles are functionalized with thioaniline electropolymerizable units and mercaptophenyl boronic acid ligands. Flavin adenine dinucleotide (FAD) is linked to the boronic acid ligands and apo-glucose oxidase, apo-GOx, is reconstituted on the FAD cofactor units to yield enzymes in a structurally-aligned configuration in respect to the Au NPs. Electropolvmerization of the enzyme-functionalized Au NPs on a thioaniline-modified Au electrode yields a three-dimensional bis-aniline-crosslinked Au NPs/reconstituted glucose oxidase matrix on the electrode that reveals effective electrical contacting with the electrode.
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