期刊
ELECTROANALYSIS
卷 22, 期 24, 页码 2908-2914出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000394
关键词
Carbon nanotube; Conducting polymer; Hydrazine label; Immunoglobulin G; Immunosensor
资金
- Ministry of Education, Science and Technology [20090079376]
An amperometric immunosensor for IgG was developed by covalently immobilizing anti-IgG on multiwall carbon nanotube-embedded conducting polymer, poly-5,2' : 5 '', 2 ''-terthiophene-3'-carboxylic acid (MWCNT/pTTCA). The MWCNT/pTTCA modified electrode was characterized by SEM, EIS, and XPS. A hydrazine-labeled secondary antibody-MWCNT conjugate (Hyd-MWCNT-Ab(2)) was applied for detection. Hydrazine was used as a catalyst for the reduction of hydrogen peroxide, which was monitored at -0.3 V vs. Ag/AgCl. The calibration plots showed a linear range of 0.1-10 ng/mL with a detection limit of 0.084 +/- 0.004 ng/mL. The proposed immunosensor was evaluated for clinical applications in a rabbit serum sample.
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