期刊
ELECTROANALYSIS
卷 22, 期 2, 页码 191-198出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200900275
关键词
Silver nanoparticles; Anodic stripping voltammetry; Electrochemical immunoassay; Nanoparticle dissolution; Myoglobin; Immunoassays; Voltammetry
资金
- National Physical Laboratory's Strategic Research Programme.
This work demonstrates the use of silver nanoparticles as a simple electrochemical biolabel to induce 10(6) signal enhancement. We propose it mechanism of measuring the silver nanoparticles oil a specific screen-printed planar carbon electrode, without the requirement for the harsh oxidant or toxic reagents described in prior-art for gold sol methods. The possible measurement process was validated with orthogonal techniques such as UV/Vis spectroscopy. dynamic light scattering and by anodic stripping voltammetry (ASV). The findings Were utilized to develop a novel electrochemical sandwich immunoassay where the analyte concentration is directly proportional to ASV oxidation peak of silver. This technique in the future is envisaged to form the foundation of a generic Point of Care platform. The assay was applied to cardiac marker: myoglobin with detection limit of 3 ng/mL.
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