期刊
ELECTROANALYSIS
卷 22, 期 19, 页码 2235-2244出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000148
关键词
Immunosensor; Passive washing; Microfluidic control; Nonspecific binding; Ag/AgCl reference electrode
资金
- National Research Foundation (NRF) [2009-0085182, 2009-0072062]
- Ministry of Education, Science and Technology (MEST) [2005-01333]
- National Research Foundation of Korea [2009-0085182, 2009-0072062] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A sensitive electrochemical immunosensing chip is presented by employing (i) selective modification of protein-resistant surfaces; (ii) fabrication of a stable Ag/AgCl reference electrode; (iii) capillary-driven microfluidic control; (iv) signal amplification by redox cycling along with enzymatic reaction. Purely capillary-driven microfluidic control is combined with electrochemical sandwich-type immunosensing procedure. Selective modification of the surfaces is achieved by chemical reactivity-controlled patterning and electrochemical deposition. Fluidic control of the immunosensing chip is achieved by spontaneous capillary-driven flows and passive washing. The detection limit for mouse IgG in the immunosensing chip is 10 pg/mL.
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