Nerve Agents Assay Using Cholinesterase Based Biosensor

Electrochemical biosensor based on electric eel acetylcholinesterase (ACME) (EC 3.1.1.7) was performed for assay of nerve agents - tabun, sarin, soman, cyclosarin, and VX. The biosensor used AChE as biorecognition element. The presence of nerve agents was accompanied by it strong inhibition of AChE activity. Enzyme activity is easily measurable by electrochemical oxidation of thiocholine created front acetylthiocholine (ATChCl) by AChE-catalyzed hydrolysis. The tested nerve agents were successfully assayed. The best limits of detection were achieved for sarin (5.88 x 10(-10) M) and VX (8.51 x 10(-10) M) after one-minute assay. The biosensor was found long term stable at low as well its laboratory temperature.